Suen, KM, Sheard, TMD, Lin, C-C orcid.org/0000-0003-3071-172X et al. (3 more authors) (2023) Expansion microscopy reveals subdomains in C. elegans germ granules. Life Science Alliance, 6 (4). e202201650. ISSN 2575-1077
Abstract
Light and electron microscopy techniques have been indispensable in the identification and characterization of liquid–liquid phase separation membraneless organelles. However, for complex membraneless organelles such as the perinuclear germ granule in C. elegans, our understanding of how the intact organelle is regulated is hampered by (1) technical limitations in confocal fluorescence imaging for the simultaneous examination of multiple granule protein markers and (2) inaccessibility of electron microscopy. We take advantage of the newly developed super resolution method of expansion microscopy (ExM) and in situ staining of the whole proteome to examine the C. elegans germ granule, the P granule. We show that in small RNA pathway mutants, the P granule is smaller compared with WT animals. Furthermore, we investigate the relationship between the P granule and two other germ granules, Mutator foci and Z granule, and show that they are located within the same protein-dense regions while occupying distinct subdomains within this ultrastructure. This study will serve as an important tool in our understanding of germ granule biology and the biological role of liquid–liquid phase separation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 Suen et al. This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Plant Cell & Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 23 May 2023 13:33 |
Last Modified: | 14 Feb 2024 10:19 |
Status: | Published |
Publisher: | Life Science Alliance |
Identification Number: | 10.26508/lsa.202201650 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:199463 |
Commentary/Response Threads
- Suen, KM, Sheard, TMD, Lin, C-C, Milonaityte, D, Jayasinghe, I and Ladbury, JE Expansion microscopy reveals subdomains in C. elegans germ granules. (deposited 23 May 2023 13:33) [Currently Displayed]