Godakumara, K., Heath, P.R. orcid.org/0000-0002-8385-1438 and Fazeli, A. (2023) Rhythm of the first language: dynamics of extracellular vesicle-based embryo–maternal communication in the pre-implantation microenvironment. International Journal of Molecular Sciences, 24 (7). 6811. ISSN 1422-0067
Abstract
One of the most critical steps in mammalian reproduction is implantation. Embryos with an impaired capacity for embryo–maternal crosstalk are thought to have a reduced potential for implantation. One agent of embryo–maternal communication is extracellular vesicles (EV). EVs are lipid bilayer-bound biological nanoparticles implicated in intercellular communication between many of the known cell types. In the current study, we isolated EVs from trophoblast analogue JAr spheroids and supplemented the EVs with receptive endometrium analogue RL95-2 cells to simulate pre-implantation embryo–maternal dialogue. The transcriptome of the endometrial cells was examined at 30 min, 4 h and 48 h intervals using Oxford Nanopore® technology. At the time points, 30 min, 4 h and 48 h, the endometrial cells showed a significantly altered transcriptome. It seems trophoblast EVs induce a swift and drastic effect on the endometrial transcriptome. The effect peaks at around 4 h of EV supplementation, indicating a generalized effect on cell physiology. Alterations are especially apparent in biological pathways critical to embryonic implantation, such as extracellular matrix–receptor interactions and cytokine–receptor interactions. These observations can be helpful in elucidating the dynamics of embryo–maternal communication in the pre-implantation period.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Keywords: | RNA-based signalling; embryo; endometrium; extracellular vesicles; nanopore; pre-implantation; trophoblast; Female; Animals; Embryo Implantation; Endometrium; Embryo, Mammalian; Extracellular Vesicles; Language; Mammals |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 26 Apr 2023 09:38 |
Last Modified: | 26 Apr 2023 09:38 |
Status: | Published |
Publisher: | MDPI AG |
Refereed: | Yes |
Identification Number: | 10.3390/ijms24076811 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:198519 |