Divan, A, Alzahrani, A, Shaik, F et al. (4 more authors) (2023) A research‐led flexible cell biology practical for biological sciences undergraduate and postgraduate degree courses. Biochemistry and Molecular Biology Education, 51 (4). pp. 394-401. ISSN 1470-8175
Abstract
A challenge in the pandemic era is to implement effective but flexible practical teaching for biological sciences courses. Such teaching needs to deliver conceptual, analytical and practical skills training while having the option to rapidly respond to health and safety issues, local regulations, staff and student concerns. In this paper, we describe a set of cell biology practicals (mini-project) that meets many of these requirements and provides flexibility in providing skills training both through online and in practical laboratory environments. We have used a human adenocarcinoma cell line A431 stably transfected with a fluorescent cell cycle reporter as a biological model to deliver training through discrete work packages encompassing cell culture, fluorescence microscopy, biochemistry and statistics. How such work packages can be modified to, an online format either partially or completely is also described. Furthermore, the activities can be adapted for teaching both undergraduate and postgraduate level courses to ensure effective skills training which is applicable to a wide range of biological degree programs and levels of study.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 The Authors. Biochemistry and Molecular Biology Education published by Wiley Periodicals LLC on behalf of International Union of Biochemistry and Molecular Biology. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
Keywords: | A431 cancer cell line; biochemistry; cell biology; cell culture; fluorescent reporter; microscopy; practical; statistics |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Cardiovascular Science (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 25 Apr 2023 14:52 |
Last Modified: | 15 Nov 2023 13:37 |
Published Version: | http://dx.doi.org/10.1002/bmb.21735 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/bmb.21735 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:198480 |