In vitro Di-ubiquitin Formation Assay and E3 Cooperation Assay

Burge, Rebecca J., Jameson, Katie H., Wilkinson, Anthony J. orcid.org/0000-0003-4577-9479 et al. (1 more author) (2022) In vitro Di-ubiquitin Formation Assay and E3 Cooperation Assay. Bio-protocol. e4547. ISSN 2331-8325

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Item Type: Article
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Funding Information: This protocol was adapted from the research published in Burge et al. (2020). We would like to thank Ubiquigent for providing a 3 month internship for RB, during which this protocol was developed. This work was supported by a Medical Research Council Studentship to RB (MRC MR/N018230/1) and the Wellcome Trust to JCM (200807/Z/16/Z). Publisher Copyright: Copyright: © 2022 The Authors; exclusive licensee Bio-protocol LLC.

Keywords: Chain formation,E2 conjugating,E3 ligase,Ubiquitin,Ubiquitination
Dates:
  • Published: 5 November 2022
Institution: The University of York
Academic Units: The University of York > Faculty of Sciences (York) > Centre for Immunology and Infection (CII) (York)
The University of York > Faculty of Sciences (York) > Biology (York)
The University of York > Faculty of Sciences (York) > Chemistry (York)
Depositing User: Pure (York)
Date Deposited: 17 Apr 2023 13:10
Last Modified: 16 Oct 2024 19:09
Published Version: https://doi.org/10.21769/BioProtoc.4547
Status: Published
Refereed: Yes
Identification Number: 10.21769/BioProtoc.4547
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