Sullivan, M.V., Henderson, A., Hand, R.A. et al. (1 more author) (2022) A molecularly imprinted polymer nanoparticle-based surface plasmon resonance sensor platform for antibiotic detection in river water and milk. Analytical and Bioanalytical Chemistry, 414 (12). pp. 3687-3696. ISSN 1618-2642
Abstract
Using a solid-phase molecular imprinting technique, high-affinity nanoparticles (nanoMIPs) selective for the target antibiotics, ciprofloxacin, moxifloxacin, and ofloxacin have been synthesised. These have been applied in the development of a surface plasmon resonance (SPR) sensor for the detection of the three antibiotics in both river water and milk. The particles produced demonstrated good uniformity with approximate sizes of 65.8 ± 1.8 nm, 76.3 ± 4.1 nm, and 85.7 ± 2.5 nm, and were demonstrated to have affinities of 36.2 nM, 54.7 nM, and 34.6 nM for the ciprofloxacin, moxifloxacin, and ofloxacin nanoMIPs, respectively. Cross-reactivity studies highlighted good selectivity towards the target antibiotic compared with a non-target antibiotic. Using spiked milk and river water samples, the nanoMIP-based SPR sensor offered comparable affinity with 66.8 nM, 33.4 nM, and 55.0 nM (milk) and 39.3 nM, 26.1 nM, and 42.7 nM (river water) for ciprofloxacin, moxifloxacin, and ofloxacin nanoMIPs, respectively, to that seen within a buffer standard. Estimated LODs for the three antibiotic targets in both milk and river water were low nM or below. The developed SPR sensor showed good potential for using the technology for the capture and detection of antibiotics from food and environmental samples.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © Springer-Verlag GmbH Germany, part of Springer Nature 2022. This is an author-produced version of a paper subsequently published in Analytical and Bioanalytical Chemistry. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Antibiotic; Fluoroquinolone; Milk; Molecularly imprinted polymer; River water; Surface plasmon resonance; Allergens; Animals; Anti-Bacterial Agents; Ciprofloxacin; Milk; Molecular Imprinting; Molecularly Imprinted Polymers; Moxifloxacin; Nanoparticles; Ofloxacin; Rivers; Surface Plasmon Resonance; Water |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 13 Apr 2023 11:17 |
Last Modified: | 13 Apr 2023 11:17 |
Status: | Published |
Publisher: | Springer Science and Business Media LLC |
Refereed: | Yes |
Identification Number: | 10.1007/s00216-022-04012-8 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:198122 |