Sheng, Q. orcid.org/0000-0001-9905-9448, Zhang, M.-Y. orcid.org/0000-0002-5707-8453, Liu, S.-M. orcid.org/0000-0003-2945-3321 et al. (13 more authors) (2023) In situ visualization of Braun’s lipoprotein on E. coli sacculi. Science Advances, 9 (3). eadd8659. ISSN 2375-2548
Abstract
Braun’s lipoprotein (Lpp) plays a major role in stabilizing the integrity of the cell envelope in Escherichia coli, as it provides a covalent cross-link between the outer membrane and the peptidoglycan layer. An important challenge in elucidating the physiological role of Lpp lies in attaining a detailed understanding of its distribution on the peptidoglycan layer. Here, using atomic force microscopy, we visualized Lpp directly on peptidoglycan sacculi. Lpp is homogeneously distributed over the outer surface of the sacculus at a high density. However, it is absent at the constriction site during cell division, revealing its role in the cell division process with Pal, another cell envelope–associated protein. Collectively, we have established a framework to elucidate the distribution of Lpp and other peptidoglycan-bound proteins via a direct imaging modality.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Escherichia coli; Peptidoglycan; Bacterial Outer Membrane Proteins; Cell Membrane; Lipoproteins |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Physics and Astronomy (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) |
Funding Information: | Funder Grant number WELLCOME TRUST (THE) 212197/Z/18/Z |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 27 Feb 2023 15:11 |
Last Modified: | 27 Feb 2023 15:11 |
Published Version: | http://dx.doi.org/10.1126/sciadv.add8659 |
Status: | Published |
Publisher: | American Association for the Advancement of Science (AAAS) |
Refereed: | Yes |
Identification Number: | 10.1126/sciadv.add8659 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:196806 |