Field, DH, White, JS, Warriner, S orcid.org/0000-0002-7589-6011 et al. (1 more author) (2023) A fluorescent photoaffinity probe for formyl peptide receptor 1 labelling in living cells. RSC Chemical Biology, 4 (3). pp. 216-222. ISSN 2633-0679
Abstract
Fluorescent ligands for G-protein coupled receptors (GPCRs) are valuable tools for studying the expression, pharmacology and modulation of these therapeutically important proteins in living cells. Here we report a fluorescent photoaffinity probe for Formyl peptide receptor 1 (FPR1), a critical component of the innate immune response to bacterial infection and a promising target in inflammatory diseases. We demonstrate that the probe binds and covalently crosslinks to FPR1 with good specificity at nanomolar concentrations in living cells and is a useful tool for visualisation and characterisation of this receptor.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 The Author(s). Published by the Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) |
Funding Information: | Funder Grant number Royal Society RG160914 Leverhulme Trust RPG-2018-030 |
Depositing User: | Symplectic Publications |
Date Deposited: | 26 Jan 2023 15:03 |
Last Modified: | 21 Jan 2025 14:51 |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Identification Number: | 10.1039/d2cb00199c |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:195435 |
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