In vitro functional analysis of gRNA sites regulating assembly of hepatitis B virus

• Patel, Nikesh
• Clark, Sam https://orcid.org/0000-0002-6865-4452
• Weiß, Eva U.
• Mata, Carlos P.
• Bohon, Jen
• Farquhar, Erik R.
• Maskell, Daniel P.
• Ranson, Neil A.
• Twarock, Reidun https://orcid.org/0000-0002-1824-2003
• Stockley, Peter G.

© The Author(s) 2021 Funding Information: We thank Prof Adam Zlotnick, Indiana University, for the gift of his Cp expression construct and advice on its purification, and Ms Leah Wells who assisted with reassembly experiments during her BSc (Honours) undergraduate research project. We thank the Medical Research Foundation for the award of a career development grant to N.P., and the UK MRC for previous grant funding to study HBV assembly (MRF-044-0002-RG-PATEL & MR/N021517/1). R.T. and P.G.S. thank The Wellcome Trust (Joint Investigator Award Nos. 110145 & 110146 to P.G.S. and R.T., respectively) for funding, and we acknowledge the financial support of The Trust of infrastructure and equipment in the Astbury Centre, University of Leeds (089311/Z/09/Z; 090932/Z/09/Z & 106692), and for their additional support, together with The University of Leeds, of the Astbury Bios-tructure Facility. R.T. acknowledges additional funding via an EPSRC Established Career Fellowship (EP/R023204/1) and a Royal Society Wolfson Fellowship (RSWF\R1\180009). Portions of this work used the XFP (17-BM) beamline at NSLS-II. Development of XFP was made possible by the National Science Foundation, Division of Biological Infrastructure (grant No. 1228549), while operations support of XFP was provided by the National Institutes of Health (grant No. P30-EB-009998). NSLS-II, a US Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Brookhaven National Laboratory, was supported under Contract No. DE-SC0012704. We thank DNA Sequencing & Services (MRC I PPU, School of Life Sciences, University of Dundee, Scotland, www.dnaseq.co.uk) for DNA sequencing.

• Published: 16 December 2021
• Accepted: 16 November 2021

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