Aleemardani, M. orcid.org/0000-0001-8261-4046, Solouk, A. orcid.org/0000-0002-8742-1056, Akbari, S. et al. (1 more author) (2023) A hydrogel–fiber–hydrogel composite scaffold based on silk fibroin with the dual‐delivery of oxygen and quercetin. Biotechnology and Bioengineering, 120 (1). pp. 297-311. ISSN 0006-3592
Abstract
Supplying sufficient oxygen within the scaffolds is one of the essential hindrances in tissue engineering that can be resolved by oxygen-generating biomaterials (OGBs). Two main issues related to OGBs are controlling oxygenation and reactive oxygen species (ROS). To address these concerns, we developed a composite scaffold entailing three layers (hydrogel-electrospun fibers-hydrogel) with antioxidant and antibacterial properties. The fibers, the middle layer, reinforced the composite structure, enhancing the mechanical strength from 4.27 ± 0.15 to 8.27 ± 0.25 kPa; also, this layer is made of calcium peroxide and silk fibroin (SF) through electrospinning, which enables oxygen delivery. The first and third layers are physical SF hydrogels to control oxygen release, containing quercetin (Q), a nonenzymatic antioxidant. This composite scaffold resulted in almost more than 40 mmHg of oxygen release for at least 13 days, and compared with similar studies is in a high range. Here, Q was used for the first time for an OGB to scavenge the possible ROS. Q delivery not only led to antioxidant activity but also stabilized oxygen release and enhanced cell viability. Based on the given results, this composite scaffold can be introduced as a safe and controllable oxygen supplier, which is promising for tissue engineering applications, particularly for bone.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 Wiley Periodicals LLC. This is an author-produced version of a paper subsequently published in Biotechnology and Bioengineering. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | calcium peroxide; composite scaffolds; oxygen-generating biomaterials; quercetin; silk fibroin; Fibroins; Hydrogels; Quercetin; Tissue Scaffolds; Antioxidants; Oxygen; Reactive Oxygen Species; Tissue Engineering; Biocompatible Materials; Silk |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 06 Jan 2023 16:57 |
Last Modified: | 28 Oct 2023 00:13 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/bit.28259 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:194995 |