Johnson, E, Clark, M, Oncul, M et al. (5 more authors) (2023) Graded spikes differentially signal neurotransmitter input in cerebrospinal fluid contacting neurons of the mouse spinal cord. iScience, 26 (1). 105914. ISSN 2589-0042
Abstract
The action potential and its all-or-none nature is fundamental to neural communication. Canonically, the action potential is initiated once voltage-activated Na⁺ channels are activated, and their rapid kinetics of activation and inactivation give rise to the action potential’s all-or-none nature. Here we demonstrate that cerebrospinal fluid contacting neurons (CSFcNs) surrounding the central canal of the mouse spinal cord employ a different strategy. Rather than using voltage-activated Na⁺ channels to generate binary spikes, CSFcNs use two different types of voltage-activated Ca²⁺ channel, enabling spikes of different amplitude. T-type Ca²⁺ channels generate small amplitude spikes, whereas larger amplitude spikes require high voltage-activated Cd²⁺-sensitive Ca²⁺ channels. We demonstrate that these different amplitude spikes can signal input from different transmitter systems; purinergic inputs evoke smaller T-type dependent spikes whereas cholinergic inputs evoke larger spikes that do not rely on T-type channels. Different synaptic inputs to CSFcNs can therefore be signaled by the spike amplitude.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Funding Information: | Funder Grant number Academy of Medical Sciences SBF002\1033 MRC (Medical Research Council) MR/V003747/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 05 Jan 2023 16:22 |
Last Modified: | 24 Jan 2023 12:39 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.isci.2022.105914 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:194855 |