Priyadarshini, S, Appukuttan, D, Victor, D et al. (2 more authors) (2022) Expression of NLRC4 Inflammasome and its Correlation with Treponema Denticola in Stage III/IV Periodontitis with Type II Diabetes mellitus. Turkish Journal of Immunology, 10 (3). pp. 162-169. ISSN 1301-109X
Abstract
To evaluate the expression of NLRC4 inflammasome and correlate with Treponema denticola (T. denticola) levels, so as to comprehend their role in mediating chronic inflammation in individuals with periodontitis (PD) and Type 2 Diabetes Mellitus (T2DM).
Materials and Methods: A total of fifty-one subjects were recruited and grouped as those systemically and periodontally healthy (PH, n=17), those systemically healthy with PD (PD, n=17), and those with T2DM with PD (PD+T2DM, n=17). Site specific probing pocket depth, clinical attachment level, plaque index, and gingival index were recorded. Thereafter, samples of subgingival plaque and gingival tissue taken with biopsy using an internal bevel incision were procured at sites evidencing the disease state. T. denticola was quantified using qPCR, and NLRC4 expression was evaluated with immunohistochemistry.
Results: Compared to the PH controls, significantly higher expression and intensity of NLRC4 inflammasome was observed in the PD and PD with T2DM groups, with a significantly greater expression in the PD+T2DM group (p<0.05). In all three groups, NLRC4 expression (mean percentage, intensity) and T. denticola levels showed a significant positive correlation (p<0.05).
Conclusion: The subgingival plaque, T. denticola levels in PD were significantly related to NLRC4 expression, both in the presence and absence of T2DM. NLRC4 activation possibly plays a role in establishing a hyperinflammatory state in diabetes mellitus modified PD.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © Copyright 2022 by the Turkish Society of Immunology. Turkish Journal of Immunology published by Galenos Publishing House. Licenced by Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) |
Keywords: | NLRC4 inflammasome, Treponema denticola, Type 2 diabetes mellitus, immunohistochemistry, qPCR, periplasmic flagella |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Restorative Dentistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 05 Jan 2023 14:17 |
Last Modified: | 25 Jun 2023 23:11 |
Status: | Published |
Publisher: | Turkish Society of Immunology |
Identification Number: | 10.4274/tji.galenos.2022.63497 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:194356 |