Hoysted, G., Field, K. orcid.org/0000-0002-5196-2360, Sinanaj, B. et al. (3 more authors) (2023) Direct nitrogen, phosphorus and carbon exchanges between Mucoromycotina ‘fine root endophyte’ fungi and a flowering plant in novel monoxenic cultures. New Phytologist, 238 (1). pp. 70-79. ISSN 0028-646X
Abstract
Most plants form mycorrhizal associations with mutualistic soil fungi. Through these partnerships, resources are exchanged including photosynthetically-fixed carbon for fungal-acquired nutrients. Recently, it was shown that the diversity of associated fungi is greater than previously assumed, extending to Mucoromycotina fungi. These Mucoromycotina ‘fine root endophytes’ (MFRE) are widespread and generally co-colonise plant roots together with Glomeromycotina ‘coarse’ arbuscular mycorrhizal fungi (AMF). Until now, this co-occurrence has hindered determination of the direct function of MFRE symbiosis.
To overcome this major barrier, we developed new techniques for fungal isolation and culture and established the first monoxenic in vitro cultures of MFRE colonising a flowering plant, clover. Using radio- and stable-isotope tracers in these in vitro systems, we measured transfer of 33P, 15N and 14C between MFRE hyphae and the host plant.
Our results provide the first unequivocal evidence that MFRE fungi are nutritional mutualists with a flowering plant by showing that clover gained both 15N and 33P tracers directly from the fungus in exchange for plant-fixed C in the absence of other microorganisms.
Our findings and methods pave the way for a new era in mycorrhizal research, firmly establishing Mucoromycotina ‘fine root endophytes’ as both mycorrhizal and functionally important in terrestrial ecosystems.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. This is an open access article under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/4.0/) which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) |
Funding Information: | Funder Grant number European Research Council 865225 NATURAL ENVIRONMENT RESEARCH COUNCIL NE/S009663/1 Biotechnology and Biological Sciences Research Council BB/M026825/1 Natural Environment Research Council NE/N00941X/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 28 Nov 2022 15:07 |
Last Modified: | 27 Sep 2024 14:39 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1111/nph.18630 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:193804 |