Brameshuber, M, Klotzsch, E, Ponjavic, A orcid.org/0000-0002-7561-1127 et al. (1 more author) (2022) Understanding immune signaling using advanced imaging techniques. Biochemical Society Transactions, 50 (2). pp. 853-866. ISSN 0300-5127
Abstract
Advanced imaging is key for visualizing the spatiotemporal regulation of immune signaling which is a complex process involving multiple players tightly regulated in space and time. Imaging techniques vary in their spatial resolution, spanning from nanometers to micrometers, and in their temporal resolution, ranging from microseconds to hours. In this review, we summarize state-of-the-art imaging methodologies and provide recent examples on how they helped to unravel the mysteries of immune signaling. Finally, we discuss the limitations of current technologies and share our insights on how to overcome these limitations to visualize immune signaling with unprecedented fidelity.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2022 The Author(s).This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
Keywords: | biophysics, confocal microscopy, lattice light sheet microscopy, single molecule localization microscopy, super-resolution imaging, widefield microscopy |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics |
Funding Information: | Funder Grant number Academy of Medical Sciences SBF006\1138 |
Depositing User: | Symplectic Publications |
Date Deposited: | 08 Dec 2022 16:17 |
Last Modified: | 08 Dec 2022 16:17 |
Status: | Published |
Publisher: | Portland Press Ltd |
Identification Number: | 10.1042/bst20210479 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:193797 |
Download
Filename: Understanding immune signaling using advanced imaging techniques.pdf
Licence: CC-BY 4.0