Anna, Anna, Calle, Beatriz, Rizou, Tatiana et al. (26 more authors) (2022) Cell-specific bioorthogonal tagging of glycoproteins. Nature Communications. 6237. ISSN 2041-1723
Abstract
Altered glycoprotein expression is an undisputed corollary of cancer development. Understanding these alterations is paramount but hampered by limitations underlying cellular model systems. For instance, the intricate interactions between tumour and host cannot be adequately recapitulated in monoculture of tumour-derived cell lines. More complex co-culture models usually rely on sorting procedures for proteome analyses and rarely capture the details of protein glycosylation. Here, we report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway that transforms bioorthogonally tagged sugars into the corresponding nucleotide-sugars. Only transfected cells incorporate bioorthogonal tags into glycoproteins in the presence of non-transfected cells. We employ BOCTAG as an imaging technique and to annotate cell-specific glycosylation sites in mass spectrometry-glycoproteomics. We demonstrate application in co-culture and mouse models, allowing for profiling of the glycoproteome as an important modulator of cellular function.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © The Author(s) 2022 |
Dates: |
|
Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 07 Oct 2022 11:10 |
Last Modified: | 17 Dec 2024 00:24 |
Published Version: | https://doi.org/10.1038/s41467-022-33854-0 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1038/s41467-022-33854-0 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:191833 |