Levenstein, M.A., Robertson, K., Turner, T.D. et al. (9 more authors) (2022) Serial small- and wide-angle X-ray scattering with laboratory sources. IUCrJ, 9 (5). pp. 538-543. ISSN 2052-2525
Abstract
Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilities, which are often oversubscribed and have long waiting times. Here, we explore the potential of state-of-the-art laboratory X-ray systems to perform comparable analyses when coupled to micro- and millifluidic sample environments. Our results demonstrate that commercial small- and wide-angle X-ray scattering (SAXS/WAXS) instruments and X-ray diffractometers are ready to access samples and timescales (≳5 ms) relevant to many processes in materials science including the preparation of pharmaceuticals, nanoparticles and functional crystalline materials. Tests of different X-ray instruments highlighted the importance of the optical configuration and revealed that serial WAXS/XRD analysis of the investigated samples was only possible with the higher flux of a microfocus setup. We expect that these results will also stimulate similar developments for structural biology.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2022 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0) |
Keywords: | serial SAXS; serial WAXS; rapid structural analysis; crystallization; microfluidics |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Funding Information: | Funder Grant number ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL EP/M028437/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL EP/R018820/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 11 Oct 2022 14:02 |
Last Modified: | 11 Oct 2022 14:02 |
Status: | Published |
Publisher: | International Union of Crystallography (IUCr) |
Refereed: | Yes |
Identification Number: | 10.1107/s2052252522007631 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:191420 |