Tonge, J.J., Keevil, B.G., Craig, J.N. et al. (3 more authors) (2022) Main salivary steroid collection in children under conditions replicating home sampling. Journal of Clinical Endocrinology and Metabolism, 107 (11). dgac419. pp. 3128-3136. ISSN 0021-972X
Abstract
Objective
Measurement of salivary glucocorticoids is an accepted method for testing adrenal function but there is little data on stability during home collection. Current salivary collection techniques require active participation or present a choking hazard and are unsuitable for young children. We sought to: compare different salivary collection methods; assess stability of salivary glucocorticoids under conditions replicating home collection; assess patient tolerability and caregiver acceptability of a salivary collection device for young children, a swab encased in an infant pacifier (SaliPac™).
Methods
Six healthy adults collected salivary samples using a Salivette® Cortisol, passive drool and SalivaBio at night, waking and 3pm for five days. Time to collect 1ml saliva using the SalivaBio and SaliPac and caregiver acceptability were assessed in 30 children <6 years. Saliva was stored at 4°C, room temperature and 50°C for 24, 48, 72 hours and a week to replicate potential postage conditions. Salivary cortisol and cortisone concentrations were measured by mass spectrometry.
Results
There was no difference in salivary glucocorticoid concentrations using the three collection methods. Salivary cortisol and cortisone were stable for 72 hours at room temperature and 4°C, and repeated freeze-thaw cycles did not cause significant degradation. In children <6 years the SalivaBio and SaliPac were well tolerated and collected sufficient saliva for salivary steroid analysis in <4 minutes.
Conclusions
Salivette, passive drool and SalivaBio collect samples with comparable salivary cortisol and cortisone concentrations, which are stable under conditions replicating home collection. SaliPac is an acceptable device for salivary sampling in young children.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2022. For the purpose of open access, the author has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising (http://creativecommons.org/licenses/by/4.0) |
Keywords: | Adrenal insufficiency; Salivary; Cortisol; Cortisone; Stability; Collection device |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Sheffield Teaching Hospitals |
Funding Information: | Funder Grant number UK RESEARCH AND INNOVATION MR/W002795/1 SOCIETY FOR ENDOCRINOLOGY UNSPECIFIED |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 17 Aug 2022 13:13 |
Last Modified: | 02 Feb 2023 11:46 |
Status: | Published |
Publisher: | The Endocrine Society |
Refereed: | Yes |
Identification Number: | 10.1210/clinem/dgac419 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:189970 |