Leva-Bueno, J orcid.org/0000-0002-3859-986X, Meuskens, I orcid.org/0000-0002-5103-1566, Linke, D orcid.org/0000-0003-3150-6752 et al. (2 more authors) (2022) A novel, proof-of-concept electrochemical impedimetric biosensor based on extracellular matrix protein–adhesin interaction. Sensors & Diagnostics, 1 (5). pp. 1003-1013. ISSN 2635-0998
Abstract
This work presents a novel perspective for electrochemical biosensors based on the detection of the interaction between extracellular matrix proteins and bacterial adhesins. Two types of impedimetric biosensors using collagen as a bioreceptor were investigated for their ability to detect pathogen adhesion using a well-characterised system of collagen-binding to recombinant E. coli expressing adhesin. First, a collagen–polymer-matrix-based biosensor was shown to detect whole bacteria in buffer media over the range 8 × 105 cfu to 8 × 107 cfu in a 10 μL sample. Second, a step-by-step full optimised biosensor based on direct collagen attachment showed bacterial detection over the range of 8 × 104 cfu to 8 × 107 cfu in a 10 μL sample. Electrochemical impedance spectroscopy and cyclic voltammetry were employed to assess the binding, which was corroborated by fluorescence binding assays. Up to now, a wide range of bioreceptors have been used in biosensor fabrication, including antibodies, oligonucleotides, phages and molecularly imprinted polymers. In this study, we show that extracellular matrix proteins can also be used for whole pathogen recognition by binding to adhesins.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 The Author(s). This is an open access article under the terms of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 15 Aug 2022 13:37 |
Last Modified: | 15 Jan 2025 14:26 |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Identification Number: | 10.1039/d2sd00075j |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:189965 |