Zitzmann, Frederik L., Ward, Ewan and Matharu, Avtar S. orcid.org/0000-0002-9488-565X (2022) Use of Carbotrace 480 as a Probe for Cellulose and Hydrogel Formation from Defibrillated Microalgae. Gels. 383. ISSN 2310-2861
Abstract
Carbotrace 480 is a commercially available fluorescent optotracer that specifically binds to cellulose’s glycosidic linkages. Herein, the use of Carbotrace 480 is reported as an analytical tool for linking cellulose content to hydrogel formation capability in defibrillated celluloses obtained from proprietary microalgae. Defibrillated celluloses obtained from acid-free hydrothermal microwave processing at low temperature (160 °C) showed poor hydrogel formation attributed to a low cellulose concentration as evidenced through the lack of Carbotrace fluorescence. High temperature (220 °C) processing afforded reasonable gels commensurate with a higher cellulose loading and stronger response to Carbotrace.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Funding Information: We would like to thank Jo Marrison and Grant Calder at the Department of Biology, University of York, UK, for running all the samples on the confocal laser microscope as well as putting a lot of time into editing all the spectra. Furthermore, we thank Donal McGee, Senior Microalgal Scientist at AlgaeCytes, Kent, UK, for kindly providing the proprietary microalgae used in this paper. Finally, thanks to John Bergqvist, Research Engineer at Ebba Biotech, Stockholm, Sweden, for providing the Carbotrace as well as many scientific discussions. We also thank the reviewers for providing valuable feedback to our work and suggesting important experimental evidence for extending the current work in the future. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
Keywords: | Carbotrace 480,defibrillated celluloses,hydrogels,microalgae |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 20 Jul 2022 08:30 |
Last Modified: | 16 Oct 2024 18:35 |
Published Version: | https://doi.org/10.3390/gels8060383 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.3390/gels8060383 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:189166 |