Badrinarayanan, Anjana and Leake, Mark C orcid.org/0000-0002-1715-1249 (2022) Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance of Chromosome (SMC) Complex in Live Escherichia coli Bacteria. Methods in Molecular Biology. pp. 31-41. ISSN 1064-3745
Abstract
MukBEF, a structural maintenance of chromosome (SMC) complex, is an important molecular machine for chromosome organization and segregation in Escherichia coli. Fluorescently tagged MukBEF forms distinct spots (or "foci") composed of molecular assemblies in the cell, where it is thought to carry out most of its chromosome-associated activities. Here, we outline the technique of fluorescence recovery after photobleaching (FRAP) as a method to study the properties of YFP-tagged MukB in fluorescent foci. This method can provide important insight into the dynamics of MukB on DNA and be used to study its biochemical properties in vivo.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details |
Keywords: | Chromosomal Proteins, Non-Histone/genetics,Chromosomes,Escherichia coli/chemistry,Escherichia coli Proteins/chemistry,Fluorescence Recovery After Photobleaching,Repressor Proteins/genetics |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Physics (York) |
Depositing User: | Pure (York) |
Date Deposited: | 13 Jul 2022 10:50 |
Last Modified: | 02 Apr 2025 23:24 |
Published Version: | https://doi.org/10.1007/978-1-0716-2221-6_4 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1007/978-1-0716-2221-6_4 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:189055 |