Shashkova, Sviatlana, Nyström, Thomas and Leake, Mark C orcid.org/0000-0002-1715-1249 (2022) Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells. Methods in Molecular Biology. pp. 183-190. ISSN 1064-3745
Abstract
Proteins are one of the key components of cellular life that play a crucial role in most biological processes. Therefore, quantification of protein copy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details |
Keywords: | Carrier Proteins/metabolism,DNA Copy Number Variations,Histone Deacetylases/metabolism,Proteins/metabolism,Saccharomyces cerevisiae/genetics,Saccharomyces cerevisiae Proteins/genetics |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Physics (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 13 Jul 2022 10:40 |
Last Modified: | 16 Oct 2024 18:33 |
Published Version: | https://doi.org/10.1007/978-1-0716-2221-6_14 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1007/978-1-0716-2221-6_14 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:189053 |