Kingston, NJ, Grehan, K orcid.org/0000-0003-3976-756X, Snowden, JS orcid.org/0000-0001-7857-0634 et al. (5 more authors) (2022) Development of an Enzyme-Linked Immunosorbent Assay for Detection of the Native Conformation of Enterovirus A71. mSphere. ISSN 2379-5042
Abstract
Enterovirus A71 (EVA71) is a medically important virus that is commonly associated with hand, foot, and mouth disease (HFMD). It is responsible for periodic outbreaks, resulting in significant economic impact and loss of life. Vaccination offers the potential to control future outbreaks, and vaccine development has been increasingly the focus of global research efforts. However, antigenic characterization of vaccine candidates is challenging because there are few tools to characterize the different antigenic forms of the virus. As with other picornaviruses, EVA71 virions exist in two antigenic states, native (NAg) and expanded (HAg). It is likely that the composition of vaccines, in terms of the proportions of NAg and HAg, will be important for vaccine efficacy and batch-to-batch consistency. This paper describes the development of a single-chain fused variable (scFv) domain fragment and the optimization of a sandwich enzyme-linked immunosorbent assay (ELISA) for the specific detection of the NAg conformation of EVA71. NAg specificity of the scFv was demonstrated using purified EVA71, and conversion of NAg to HAg by heating resulted in a loss of binding. We have thus developed an effective tool for characterization of the specific antigenic state of EVA71.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | enterovirus A71; sandwich ELISA; scFv; EVA71 |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Molecular Virology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 17 Jun 2022 12:05 |
Last Modified: | 17 Jun 2022 12:05 |
Status: | Published online |
Publisher: | American Society for Microbiology |
Identification Number: | 10.1128/msphere.00088-22 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:188071 |