Kingston, NJ, Shegdar, M, Snowden, JS orcid.org/0000-0001-7857-0634 et al. (5 more authors) (2022) Thermal stabilization of enterovirus A 71 and production of antigenically stabilized empty capsids. Journal of General Virology, 103 (8). 001771. ISSN 0022-1317
Abstract
Enterovirus A71 (EVA71) infection can result in paralysis and may be fatal. In common with other picornaviruses, empty capsids are produced alongside infectious virions during the viral lifecycle. These empty capsids are antigenically indistinguishable from infectious virus, but at moderate temperatures they are converted to an expanded conformation. In the closely related poliovirus, native and expanded antigenic forms of particle have different long-term protective efficacies when used as vaccines. The native form provides long-lived protective immunity, while expanded capsids fail to generate immunological protection. Whether this is true for EVA71 remains to be determined. Here, we selected an antigenically stable EVA71 virus population using successive rounds of heating and passage and characterized the antigenic conversion of both virions and empty capsids. The mutations identified within the heated passaged virus were dispersed across the capsid, including at key sites associated with particle expansion. The data presented here indicate that the mutant sequence may be a useful resource to address the importance of antigenic conformation in EVA71 vaccines.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
Keywords: | EVA71, empty capsids, stabilization, infantile paralysis |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Molecular Virology (Leeds) |
Funding Information: | Funder Grant number MRC (Medical Research Council) MR/P022626/1 Wellcome Trust 204825/Z/16/Z Wellcome Trust 102174/B/13/Z |
Depositing User: | Symplectic Publications |
Date Deposited: | 06 Jun 2022 09:48 |
Last Modified: | 13 Oct 2022 14:04 |
Status: | Published |
Publisher: | Microbiology Society |
Identification Number: | 10.1099/jgv.0.001771 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:187592 |