Bublitz, D.C., Chadwick, G.L., Magyar, J.S. et al. (8 more authors) (2019) Peptidoglycan production by an insect-bacterial mosaic. Cell, 179 (3). 703-712.e7. ISSN 0092-8674
Abstract
Peptidoglycan (PG) is a defining feature of bacteria, involved in cell division, shape, and integrity. We previously reported that several genes related to PG biosynthesis were horizontally transferred from bacteria to the nuclear genome of mealybugs. Mealybugs are notable for containing a nested bacteria-within-bacterium endosymbiotic structure in specialized insect cells, where one bacterium, Moranella, lives in the cytoplasm of another bacterium, Tremblaya. Here we show that horizontally transferred genes on the mealybug genome work together with genes retained on the Moranella genome to produce a PG layer exclusively at the Moranella cell periphery. Furthermore, we show that an insect protein encoded by a horizontally transferred gene of bacterial origin is transported into the Moranella cytoplasm. These results provide a striking parallel to the genetic and biochemical mosaicism found in organelles, and prove that multiple horizontally transferred genes can become integrated into a functional pathway distributed between animal and bacterial endosymbiont genomes.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) |
Funding Information: | Funder Grant number BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL BB/N000951/1 Biotechnology and Biological Sciences Research Council 2058718 MEDICAL RESEARCH COUNCIL MR/S009272/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 18 May 2022 14:06 |
Last Modified: | 10 Feb 2023 14:13 |
Status: | Published |
Publisher: | Elsevier BV |
Refereed: | Yes |
Identification Number: | 10.1016/j.cell.2019.08.054 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:186959 |
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Licence: CC-BY-NC-ND 4.0