Gautrey, S.L. and Simons, M.J.P. orcid.org/0000-0001-7406-7708 (2022) Amino acid availability is not essential for lifespan extension by dietary restriction in the fly. Journals of Gerontology, Series A, 77 (11). pp. 2181-2185. ISSN 1079-5006
Abstract
Dietary restriction (DR) is one of the most potent ways to extend health- and lifespan. Key progress in understanding the mechanisms of DR, and ageing more generally, was made when dietary protein, and more specifically essential amino acids (EAA), were identified as the dietary component to restrict to obtain DR’s health and lifespan benefits. This role of dietary amino acids has influenced work on ageing mechanisms, especially in nutrient sensing, e.g. Tor and insulin(-like) signalling networks. Experimental biology in Drosophila melanogaster has been instrumental in generating and confirming the hypothesis that EAA availability is important in ageing. Here, we expand on previous work testing the involvement of EAA in DR through large scale (N=6,238) supplementation experiments across four diets and two genotypes in female flies. Surprisingly, we find that EAA are not essential to DR’s lifespan benefits. Importantly, we do identify the fecundity benefits of EAA supplementation suggesting the supplemented EAA were bioavailable. Furthermore, we find that the effects of amino acids on lifespan vary by diet and genetic line studied and that at our most restricted diet fecundity is constrained by other nutrients than EAA. We suggest that DR for optimal health is a concert of nutritional effects, orchestrated by genetic, dietary and other environmental interactions. Our results question the universal importance of amino acid availability in the biology of ageing and DR.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2022. Published by Oxford University Press on behalf of The Gerontological Society of America. This is an author-produced version of a paper subsequently published in The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | ageing; diet; drosophila; nutrients; reaction norm |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) |
Funding Information: | Funder Grant number ACADEMY OF MEDICAL SCIENCES SBF004\1085 WELLCOME TRUST (THE) 216405/Z/19/Z |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 13 May 2022 13:04 |
Last Modified: | 29 Apr 2023 00:13 |
Status: | Published |
Publisher: | Oxford University Press (OUP) |
Refereed: | Yes |
Identification Number: | 10.1093/gerona/glac100 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:186813 |