Alix, J. orcid.org/0000-0001-8391-9749, Plesia, M., Lloyd, G.R. et al. (9 more authors) (2022) Rapid identification of human muscle disease with fibre optic Raman spectroscopy. Analyst, 2022 (11). 147. pp. 2533-2540. ISSN 0003-2654
Abstract
The diagnosis of muscle disorders (“myopathies”) can be challenging and new biomarkers of disease are required to enhance clinical practice and research. Despite advances in areas such as imaging and genomic medicine, muscle biopsy remains an important but time consuming investigation. Raman spectroscopy is a vibrational spectroscopy application that could provide a rapid analysis of muscle tissue, as it requires no sample preparation and is simple to perform. Here, we investigated the feasibility of using a miniaturised, portable fibre optic Raman system for the rapid identification of muscle disease. Samples were assessed from 29 patients with a final clinico-pathological diagnosis of a myopathy and 17 patients in whom investigations and clinical follow-up excluded myopathy. Multivariate classification techniques achieved accuracies ranging between 71-80%. To explore the potential of Raman spectroscopy to identify different myopathies, patients were subdivided into mitochondrial and non-mitochondrial myopathy groups. Classification accuracies were between 78 – 89%. Observed spectral changes were related to changes in protein structure. These data indicate fibre optic Raman spectroscopy is a promising technique for the rapid identification of muscle disease that could provide real time diagnostic information. The application of fibre optic Raman technology raises the prospect of in vivo bedside testing for muscle diseases which would significantly streamline the diagnostic pathway of these disorders.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 The Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/). |
Keywords: | Clinical Research; Rare Diseases; Brain Disorders; 4.1 Discovery and preclinical testing of markers and technologies; 4.2 Evaluation of markers and technologies; Musculoskeletal |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Sheffield Teaching Hospitals |
Funding Information: | Funder Grant number ACADEMY OF MEDICAL SCIENCES nan |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 04 May 2022 15:56 |
Last Modified: | 24 Nov 2022 15:02 |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Refereed: | Yes |
Identification Number: | 10.1039/d1an01932e |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:186254 |