Moore, A., Chinnaiya, K., Kim, D.W. et al. (11 more authors) (2022) Loss of function of the neural cell adhesion molecule NrCAM regulates differentiation, proliferation and neurogenesis in early postnatal hypothalamic tanycytes. Frontiers in Neuroscience, 16. 832961. ISSN 1662-4548
Abstract
Hypothalamic tanycytes are neural stem and progenitor cells, but little is known of how they are regulated. Here we provide evidence that the cell adhesion molecule, NrCAM, regulates tanycytes in the adult niche. NrCAM is strongly expressed in adult mouse tanycytes. Immunohistochemical and in situ hybridization analysis revealed that NrCAM loss of function leads to both a reduced number of tanycytes and reduced expression of tanycyte-specific cell markers, along with a small reduction in tyrosine hydroxylase-positive arcuate neurons. Similar analyses of NrCAM mutants at E16 identify few changes in gene expression or cell composition, indicating that NrCAM regulates tanycytes, rather than early embryonic hypothalamic development. Neurosphere and organotypic assays support the idea that NrCAM governs cellular homeostasis. Single-cell RNA sequencing (scRNA-Seq) shows that tanycyte-specific genes, including a number that are implicated in thyroid hormone metabolism, show reduced expression in the mutant mouse. However, the mild tanycyte depletion and loss of markers observed in NrCAM-deficient mice were associated with only a subtle metabolic phenotype.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 Moore, Chinnaiya, Kim, Brown, Stewart, Robins, Dowsett, Muir, Travaglio, Lewis, Ebling, Blackshaw, Furley and Placzek. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Keywords: | tanycyte; NrCAM; neural cell adhesion molecules; scRNA seq; radial glia; astrocytes; hypothalamus; neurogenesis |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) |
Funding Information: | Funder Grant number WELLCOME TRUST (THE) 212247/Z/18/Z |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 26 Apr 2022 13:00 |
Last Modified: | 11 Feb 2023 02:32 |
Status: | Published |
Publisher: | Frontiers Media |
Refereed: | Yes |
Identification Number: | 10.3389/fnins.2022.832961 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:185782 |