Wollman, Adam J M orcid.org/0000-0002-5501-8131, Kioumourtzoglou, Dimitrios, Ward, Rebecca et al. (2 more authors) (2022) Large scale, single-cell FRET-based glucose uptake measurements within heterogeneous populations. iScience. 104023. p. 104023. ISSN 2589-0042
Abstract
Fluorescent biosensors are powerful tools allowing the concentration of metabolites and small molecules, and other properties such as pH and molecular crowding to be measured inside live single cells. The technology has been hampered by lack of simple software to identify cells and quantify biosensor signals in single cells. We have developed a new software package, FRETzel, to address this gap and demonstrate its use by measuring insulin-stimulated glucose uptake in individual fat cells of varying sizes for the first time. Our results support the long-standing hypothesis that larger fat cells are less sensitive to insulin than smaller ones, a finding that has important implications for the battle against type 2 diabetes. FRETzel has been optimized using the messy and crowded environment of cultured adipocytes, demonstrating its utility for quantification of FRET biosensors in a wide range of other cell types, including fibroblasts and yeast via a simple user-friendly quantitative interface.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Crown Copyright © 2022. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Physics (York) |
Depositing User: | Pure (York) |
Date Deposited: | 06 Apr 2022 15:50 |
Last Modified: | 21 Nov 2024 00:45 |
Published Version: | https://doi.org/10.1016/j.isci.2022.104023 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.isci.2022.104023 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:185508 |