Esther Elizabeth Grace, C., Briget Mary, M., Vaidyanathan, S. orcid.org/0000-0003-4137-1230 et al. (1 more author) (2022) Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 270. 120830. ISSN 1386-1425
Abstract
Two green microalgae species Monoraphidium contortum (M. contortum) and Chlamydomonas sp. that were identified to accumulate lipids were subjected to four different nutrient treatments (NP1-NP4), ranging in nitrate (0.05–5 mM N) and phosphate (2.8–264 μM P) concentrations, at a fixed N:P ratio of ∼18. The effect of nutrient variation on lipid productivity in the species was investigated using second derivative (SD) FTIR and Raman spectroscopy of algal biomass. SD spectral analysis revealed high production of lipid in the form of hydrocarbons (CH) (3000–2800 cm−1), triacylglycerides (TAGs)(∼1740 cm−1), saturated (SFA)(∼1440 cm−1), and unsaturated fatty acids (UFA)(∼3010 cm−1) for the nutrient deplete condition (NP1) in both species. Changes in signals attributed to lipids in proportion to other biochemical components were consistent with physiological changes expected from nutrient depletion. Relative signal intensities for lipids showed a significant increase in NP1, in particular, CH, TAGs in relation to protein signals (in SD-FTIR), and SFA, UFA in relation to carotenoid signals (in SD-Raman). PCA performed on the negative spectral values of the SD-FTIR and SD-Raman data for the four NP treatments enabled discrimination not only between the species but also between the NP treatments and the timing of harvest. M. contortum was found to contain a relatively higher proportion of CH, TAGs, SFA, and UFA compared to Chlamydomonas sp. Peak areas from the negative SD spectra, informed by PCA analysis, enabled capturing quantifiable changes in a manner that is consistent with known microalgal physiology. SD-FTIR and SD-Raman spectroscopy have been shown to possess superior potential to capture relevant microalgal physiological changes.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 Elsevier B.V. This is an author produced version of a paper subsequently published in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. Uploaded in accordance with the publisher's self-archiving policy. Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
Keywords: | Biodiesel; Chlamydomonas; Hydrocarbons; Monoraphidium; PCA; Triacylglycerides (TAGs); Vibrational spectroscopy; Biofuels; Biomass; Fatty Acids; Lipids; Microalgae; Nutrients; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) |
Funding Information: | Funder Grant number Biotechnology and Biological Sciences Research Council BB/K020633/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 24 Mar 2022 11:57 |
Last Modified: | 29 Dec 2022 01:13 |
Status: | Published |
Publisher: | Elsevier BV |
Refereed: | Yes |
Identification Number: | 10.1016/j.saa.2021.120830 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:185100 |
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