Nampi, PP orcid.org/0000-0001-8702-0060, Vakurov, A, Saha, S et al. (2 more authors) (2022) Surface modified hexagonal upconversion nanoparticles for the development of competitive assay for biodetection. Biomaterials Advances, 136. 212763. ISSN 2772-9508
Abstract
Up-conversion nanoparticles (UCNPs) of sodium yttrium fluoride with ytterbium and erbium ions as sensitizer and activator (β-NaYF4/Yb3+/Er3+) have been synthesised by a solvo-thermal method. The synthesised particles were found to be highly uniform in size (~50 nm) and of hexagonal crystal phase producing strong up-conversion luminescence dominated in the green wavelength region. During the synthesis, luminescence properties of the reaction mixture were monitored at regular intervals to ensure the required particle size distribution and luminescence efficiency. The hydrophobic particles thus obtained were modified by coating with silica, yielding particles that were stable in aqueous media. The silica coated UCNPs were further modified with maleimide-polyethylene glycol-silane (mal-PEG-silane) to provide thiol reactive surface groups. The silanized, maleimide-bearing UCNPs were effective for conjugating to reductively-cleaved half antibodies against ofloxacin, a veterinary antibiotic, to produce luminescent nanobiosensors for its detection and quantification. The speed and minimum detection concentration (~10 nM) that we report for a competitive assay of ofloxacin in this study is promising for developing sensors for this and other biomolecules.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license https://creativecommons.org/licenses/by/4.0/ |
Keywords: | Up-conversion nanoparticles; Photoluminescence; Detection; Ofloxacin; Competitive assay |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 28 Mar 2022 14:52 |
Last Modified: | 25 Jun 2023 22:56 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.bioadv.2022.212763 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:185043 |