Roper, BWR orcid.org/0000-0002-9897-4254, Al-Sayejh, B, Al-Aufi,, A et al. (7 more authors) (2022) Purification and analysis of circulating lipid particles. In: Methods in Molecular Biology. Methods in Molecular Biology . Springer Nature
Abstract
Lipid particles found in circulating extracellular fluids such as blood or lymph are essential for cellular homeostasis, metabolism and survival. Such particles provide essential lipids and fats which enable cells to synthesize new membranes and regulate different biochemical pathways. Imbalance in lipid particle metabolism can cause pathological states such as atherosclerosis. Here, elevated low-density lipoprotein (LDL) accumulation leads to fat-filled lesions or plaques in arterial walls. In this chapter, we provide a detailed set of protocols for the rapid and safe purification of lipid particles from human blood using high-speed ultracentrifugation. We provide a detailed set of assays for further analysis of the biochemical and cellular properties of these lipid particles. By combining these assays, we can better understand the complex roles of different lipid particles in normal physiology and disease pathology.
Metadata
Item Type: | Book Section |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022. This is an author produced version of a book chapter published in Atherosclerosis: Methods and Protocols. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Lipid particles; LDL; VLDL; HDL; Gradient purification; SDS-PAGE; Human umbilical vein endothelial cells (HUVECs); Human embryonic kidney 293T (HEK293T) cells |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Cardiovascular Science (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) > Clinical & Population Science Dept (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 21 Mar 2022 14:00 |
Last Modified: | 22 Feb 2024 11:49 |
Status: | Published |
Publisher: | Springer Nature |
Series Name: | Methods in Molecular Biology |
Identification Number: | 10.1007/978-1-0716-1924-7_12 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:184962 |