Anton, A. orcid.org/0000-0002-1356-4513, Mead, R.J. orcid.org/0000-0002-3207-0068, Shaw, P.J. orcid.org/0000-0002-8925-2567 et al. (6 more authors) (2022) Assessment of the precision in measuring glutathione at 3 T with a MEGA-PRESS sequence in primary motor cortex and occipital cortex. Journal of Magnetic Resonance Imaging, 55 (2). pp. 435-442. ISSN 1053-1807
Abstract
Background
Glutathione (GSH) is an important brain antioxidant and a number of studies have reported its measurement by edited and nonedited localized 1H spectroscopy techniques within a range of applications in healthy volunteers and disease states. Good test–retest reproducibility is key when assessing the efficacy of treatments aimed at modulating GSH levels within the central nervous system or when noninvasively assessing changes in GSH content over time.
Purpose
To evaluate the intraday (in vitro and in vivo) and 1-month apart (in vivo) test–retest reproducibility of GSH measurements from GSH-edited MEGA-PRESS acquisitions at 3 T in a phantom and in the brain of a cohort of middle-aged and older healthy volunteers.
Study Type
Prospective.
Subjects/Phantoms
A phantom containing physiological concentrations of GSH and metabolites with overlapping spectral signatures and 10 healthy volunteers (4 F, 6 M, 55 ± 14 years old).
Field strength/Sequence
GSH-edited spectra were acquired at 3 T using the MEGA-PRESS sequence.
Assessment
The phantom was scanned twice and the healthy subjects were scanned three times (on two separate days, 1 month apart). GSH was quantified from each acquisition, with the in vivo voxels placed at the primary motor cortex (PMC) and the occipital cortex (OCC).
Statistical Tests
Mean coefficients of variation (CV) were used to assess short-term (in vitro and in vivo) and longer-term (in vivo) test–retest reproducibility.
Results
In vitro, the CV was 2.3%. In vivo, the mean intraday CV was 3.3% in the PMC and 2.4% in the OCC, while the CVs at 1 month apart were 4.6% in the PMC and 7.8% in the OCC.
Data Conclusion
GSH-edited MEGA-PRESS spectroscopy allows measurement of GSH with excellent precision.
Evidence Level
1
Technical Efficacy
Stage 2
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 International Society for Magnetic Resonance in Medicine. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Sheffield Teaching Hospitals |
Funding Information: | Funder Grant number Medical Research Council MR/S004920/1 National Institute for Health Research NIHRDH-IS-BRC-1215-20017 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 25 Mar 2022 09:49 |
Last Modified: | 25 Mar 2022 09:49 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/jmri.27842 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:184931 |