Mclure, RJ, Radford, SE orcid.org/0000-0002-3079-8039 and Brockwell, DJ orcid.org/0000-0002-0802-5937 (2022) High-throughput directed evolution: a golden era for protein science. Trends in Chemistry, 4 (5). pp. 378-391. ISSN 2589-5974
Abstract
Directed evolution is a robust and powerful tool for engineering new and/or improved functions in biomolecules for therapeutic and industrial applications, as well as to uncover fundamental insights into protein behaviour. It works by exploiting the principle of natural evolution and accelerating it through multiple rounds of gene diversification and selection. To evolve the desired property, an appropriate assay for the property of interest must be chosen. Here, we describe recent advances in the development of in vitro and in vivo diversification methods, as well as high-throughput assays for protein directed evolution. Using recent examples, we discuss the drawbacks and challenges of the array of diversification methods and selection assays and consider future challenges in the field.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | directed evolution; protein engineering; continuous evolution; screening; selection; mutagenesis; deep mutational scanning |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Structural Molecular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 21 Feb 2022 11:36 |
Last Modified: | 21 Mar 2023 16:26 |
Status: | Published |
Publisher: | Cell Press |
Identification Number: | 10.1016/j.trechm.2022.02.004 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:183791 |