Stupka, I, Azuma, Y, Biela, AP et al. (6 more authors) (2022) Chemically induced protein cage assembly with programmable opening and cargo release. Science Advances, 8 (1). ISSN 2375-2548
Abstract
Engineered protein cages are promising tools that can be customized for applications in medicine and nanotechnology. A major challenge is developing a straightforward strategy for endowing cages with bespoke, inducible disassembly. Such cages would allow release of encapsulated cargoes at desired timing and location. Here, we achieve such programmable disassembly using protein cages, in which the subunits are held together by different molecular cross-linkers. This modular system enables cage disassembly to be controlled in a condition-dependent manner. Structural details of the resulting cages were determined using cryo–electron microscopy, which allowed observation of bridging cross-linkers at intended positions. Triggered disassembly was demonstrated by high-speed atomic force microscopy and subsequent cargo release using an encapsulated Förster resonance energy transfer pair whose signal depends on the quaternary structure of the cage.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. This is an open access article under the terms of the Creative Commons Attribution NonCommercial License (CC BY-NC 4.0) |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 09 Feb 2022 16:31 |
Last Modified: | 09 Feb 2022 16:31 |
Status: | Published |
Publisher: | American Association for the Advancement of Science (AAAS) |
Identification Number: | 10.1126/sciadv.abj9424 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:183375 |
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