Endothelial Cell Fibrin Gel Angiogenesis Bead Assay.

Abstract

The fibrin gel angiogenesis bead assay provides a controlled in vitro setting for observing endothelial angiogenic sprouting in response to modified variables. Endothelial cells are coated onto microcarriers and embedded into a fibrin clot containing necessary growth factors. Following a 24-h incubation, endothelial sprouts are imaged using a light microscope. This method is useful for rapidly and affordably investigating the effects of genetic or chemical manipulation to endothelial function.

Metadata

Item Type:	Book Section
Authors/Creators:	Clavane, EM Taylor, HA Cubbon, RM https://orcid.org/0000-0001-7844-3600 Meakin, PJ https://orcid.org/0000-0002-8553-3863
Editors:	Benest, AV
Keywords:	Angiogenesis; Fibrin matrix; Cytodex® 3 beads; Endothelial cells; Sprouting
Dates:	Published: 1 January 2022
Institution:	The University of Leeds
Academic Units:	The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) > Discovery & Translational Science Dept (Leeds)
Funding Information:	Funder Grant number British Heart Foundation FS/13/38/33659 BBSRC (Biotechnology & Biological Sciences Research Council) BB/V014358/1
Depositing User:	Symplectic Publications
Date Deposited:	07 Feb 2022 13:35
Last Modified:	07 Feb 2022 13:35
Status:	Published
Publisher:	Springer
Series Name:	Methods in Molecular Biology
Identification Number:	10.1007/978-1-0716-2059-5_25
Related URLs:	PubMed URL
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:183274

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Endothelial Cell Fibrin Gel Angiogenesis Bead Assay.

Abstract

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