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Kemppainen, J. orcid.org/0000-0002-9140-6932, Mansour, N., Takalo, J. orcid.org/0000-0002-1638-0808 et al. (1 more author) (Submitted: 2021) High-speed imaging of light-induced photoreceptor microsaccades in compound eyes. bioRxiv. (Submitted)
Abstract
Inside compound eyes, photoreceptors contract to light changes, sharpening retinal images of the moving world in time. Current methods to measure these so-called photoreceptor microsaccades in living insects are spatially limited and technically challenging. Here, we present goniometric high-speed deep pseudopupil (GHS-DPP) microscopy to assess how the rhabdomeric insect photoreceptors and their microsaccades are organised across the compound eyes. This method enables non-invasive rhabdomere orientation mapping, whilst their microsaccades can be locally light-activated, revealing the eyes’ underlying active sampling motifs. By comparing the microsaccades in wild-type Drosophila’s open rhabdom eyes to spam-mutant eyes, reverted to an ancestral fused rhabdom state, and honeybee’s fused rhabdom eyes, we show how different eye types sample light information. These results show different ways compound eyes initiate the conversion of spatial light patterns in the environment into temporal neural signals and highlight how this active sampling can evolve with insects’ visual needs.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 The Authors. Preprint made available under a CC-BY-NC-ND 4.0 International license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
Keywords: | photoreceptor; open rhabdom; fused rhabdom; microsaccade; Drosophila; deep pseudopupil; goniometry; hyperacuity; active sampling; active vision |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 24 Jan 2022 11:57 |
Last Modified: | 24 Jan 2022 11:57 |
Status: | Submitted |
Publisher: | Cold Spring Harbor Laboratory |
Identification Number: | 10.1101/2021.07.22.453335 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:182846 |
Available Versions of this Item
- High-speed imaging of light-induced photoreceptor microsaccades in compound eyes. (deposited 24 Jan 2022 11:57) [Currently Displayed]