Grey, William George orcid.org/0000-0001-8209-5645, Chauhan, R., Piganeau, M. et al. (4 more authors) (2020) Activation of the receptor tyrosine kinase RET improves long-term hematopoietic stem cell outgrowth and potency. Blood. pp. 2535-2547. ISSN 1528-0020
Abstract
Expansion of human hematopoietic stem cells (HSCs) is a rapidly advancing field showing great promise for clinical applications. Recent evidence has implicated the nervous system and glial family ligands (GFLs) as potential drivers of hematopoietic survival and self-renewal in the bone marrow niche; how to apply this process to HSC maintenance and expansion has yet to be explored. We show a role for the GFL receptor, RET, at the cell surface of HSCs in mediating sustained cellular growth, resistance to stress, and improved cell survival throughout in vitro expansion. HSCs treated with the key RET ligand/coreceptor complex, glial-derived neurotrophic factor and its coreceptor, exhibit improved progenitor function at primary transplantation and improved long-term HSC function at secondary transplantation. Finally, we show that RET drives a multifaceted intracellular signaling pathway, including key signaling intermediates protein kinase B, extracellular signal-regulated kinase 1/2, NF-κB, and p53, responsible for a wide range of cellular and genetic responses that improve cell growth and survival under culture conditions.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 12 Jan 2022 10:00 |
Last Modified: | 02 Nov 2024 01:24 |
Published Version: | https://doi.org/10.1182/blood.2020006302 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1182/blood.2020006302 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:182447 |
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Filename: bloodbld2020006302.pdf
Description: Activation of the receptor tyrosine kinase RET improves long-term hematopoietic stem cell outgrowth and potency
Licence: CC-BY 2.5