Mazilescu, Laura I, Urbanellis, Peter, Kaths, Moritz J et al. (19 more authors) (2021) Prolonged Normothermic Ex Vivo Kidney Perfusion Is Superior to Cold Nonoxygenated and Oxygenated Machine Perfusion for the Preservation of DCD Porcine Kidney Grafts. Transplantation direct. e751. ISSN: 2373-8731
Abstract
The increased usage of marginal grafts has triggered interest in perfused kidney preservation to minimize graft injury. We used a donation after circulatory death (DCD) porcine kidney autotransplantation model to compare 3 of the most frequently used ex vivo kidney perfusion techniques: nonoxygenated hypothermic machine perfusion (non-oxHMP), oxygenated hypothermic machine perfusion (oxHMP), and normothermic ex vivo kidney perfusion (NEVKP). Methods: Following 30 min of warm ischemia, grafts were retrieved and preserved with either 16 h of non-oxHMP, oxHMP, or NEVKP (n = 5 per group). After contralateral nephrectomy, grafts were autotransplanted and animals were followed for 8 d. Kidney function and injury markers were compared between groups. Results: NEVKP demonstrated a significant reduction in preservation injury compared with either cold preservation method. Grafts preserved by NEVKP showed superior function with lower peak serum creatinine (NEVKP versus non-oxHMP versus oxHMP: 3.66 ± 1.33 mg/dL, 8.82 ± 3.17 mg/dL, and 9.02 ± 5.5 mg/dL) and more rapid recovery. The NEVKP group demonstrated significantly increased creatinine clearance on postoperative day 3 compared with the cold perfused groups. Tubular injury scores on postoperative day 8 were similar in all groups. Conclusions: Addition of oxygen during HMP did not reduce preservation injury of DCD kidney grafts. Grafts preserved with prolonged NEVKP demonstrated superior initial graft function compared with grafts preserved with non-oxHMP or oxHMP in a model of pig DCD kidney transplantation.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | Copyright © 2021 The Author(s). Transplantation Direct. Published by Wolters Kluwer Health, Inc. |
Dates: |
|
Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 07 Jan 2022 12:00 |
Last Modified: | 27 Aug 2025 13:31 |
Published Version: | https://doi.org/10.1097/TXD.0000000000001218 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1097/TXD.0000000000001218 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:182215 |
Download
Filename: Prolonged_Normothermic_Ex_Vivo_Kidney_Perfusion_Is.6.pdf
Description: Prolonged_Normothermic_Ex_Vivo_Kidney_Perfusion_Is.6
Licence: CC-BY-NC-ND 2.5