Wilson, Julie C. orcid.org/0000-0002-5171-8480, Kealy, David James orcid.org/0000-0001-9246-8134, James, Sally Rachel et al. (19 more authors) (2022) Integrated miRNA/cytokine/chemokine profiling reveals severity-associated step changes and principal correlates of fatality in COVID-19. iScience. 103672. ISSN 2589-0042
Abstract
Inflammatory cytokines and chemokines (CC) drive COVID-19 pathology. Yet, patients with similar circulating CC levels present with different disease severity. Here, we determined 171 microRNAomes from 58 hospitalized COVID-19 patients (Cohort 1) and levels of 25 cytokines and chemokines (CC) in the same samples. Combining microRNA (miRNA) and CC measurements allowed for discrimination of severe cases with greater accuracy than using miRNA or CC levels alone. Severity group-specific associations between miRNAs and COVID-19-associated CC (e.g., IL6, CCL20) or clinical hallmarks of COVID-19 (e.g., neutrophilia, hypoalbuminemia) separated patients with similar CC levels but different disease severity. Analysis of an independent cohort of 108 patients from a different center (Cohort 2) demonstrated feasibility of CC/miRNA profiling in leftover hospital blood samples with similar severe disease CC and miRNA profiles, and revealed CCL20, IL6, IL10, and miR-451a as key correlates of fatal COVID-19. These findings highlight that systemic miRNA/CC networks underpin severe COVID-19.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 The Author(s) |
Keywords: | Clinical finding,Complex system biology,Health sciences |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Mathematics (York) The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Hull York Medical School (York) |
Funding Information: | Funder Grant number MEDICAL RESEARCH COUNCIL (MRC) MR/V028448/1 |
Depositing User: | Pure (York) |
Date Deposited: | 05 Jan 2022 13:00 |
Last Modified: | 18 Dec 2024 00:20 |
Published Version: | https://doi.org/10.1016/j.isci.2021.103672 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.isci.2021.103672 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:182106 |
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Filename: PIIS2589004221016424.pdf
Description: Integrated miRNA/cytokine/chemokine profiling reveals severity-associated step changes and principal correlates of fatality in COVID-19
Licence: CC-BY 2.5