Miller, Lisa Margaret orcid.org/0000-0003-3667-3840, Simmons, Matt, Silver, Callum et al. (4 more authors) (2022) Antibiotic-functionalized Gold Nanoparticles for the Detection of Active β-lactamases. Nanoscale Advances. pp. 573-581. ISSN 2516-0230
Abstract
Antimicrobial resistance (AMR) continues to threaten the effective treatment and prevention of bacterial infections. The spread of resistant infections is accelerated by the lack of fast and cost-effective tests for the detection of AMR at the point-of-care. We aimed to address this challenge by developing a diagnostic tool to detect one of the major forms of AMR, the β-lactamase enzymes. Antibiotic-functionalized gold nanoparticles (AuNPs) have been successfully developed for the detection of β-lactamases in challenging biological media, namely undiluted urine. Furthermore, these tools are compatible with samples containing a urine sample preservative (boric acid) or hematuria (blood). The functionalized AuNPs interact with the active β-lactamases, resulting in the hydrolysis of the surface-bound antibiotics, which then inhibits binding of the AuNPs to a capture protein (a penicillin-binding protein) to indicate the presence of active β-lactamases. We successfully integrated the antibiotic-functionalized AuNPs into a new lateral flow assay (LFA), which can be used to detect active β-lactamases down to the detection limit of 11 nM. While we demonstrate the use of antibiotic-functionalized AuNPs in an LFA format to provide a novel method of detecting active β-lactamases, these functionalized AuNPs are amenable to a range of alternative diagnostic technologies and could lead to vital point-of-care diagnostics for the early detection of multi-drug resistant infections.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York > Faculty of Sciences (York) > Electronic Engineering (York) The University of York > Faculty of Sciences (York) > Physics (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Funding Information: | Funder Grant number EPSRC EP/P02324X/1 |
Depositing User: | Pure (York) |
Date Deposited: | 15 Dec 2021 15:20 |
Last Modified: | 26 Feb 2025 00:07 |
Published Version: | https://doi.org/10.1039/D1NA00635E |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1039/D1NA00635E |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:181626 |
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Description: Antibiotic-functionalized Gold Nanoparticles for the Detection of Active -lactamases