Dugan, J.M., Colominas, C., Garcia-Granada, A.-A. et al. (1 more author) (2021) Spatial control of neuronal adhesion on diamond-like carbon. Frontiers in Materials, 8. 756055.
Abstract
This study reports a route to spatial control of neuronal adhesion onto Diamond-Like Carbon (DLC) by surface functionalisation by poly (oligo-ethyleneglycol methacrylate) (pOEGMA) and consequent laser ablation to produce cell adhesive tracks. DLC can be deposited as a tough and low friction coating on implantable devices and surgical instruments and has favourable properties for use as a biomaterial. The pOEGMA surface coating renders the DLC surface antifouling and the laser ablation creates graphitised tracks on the surface. The surfaces were coated with laminin, which adhered preferentially to the ablation tracks. The patterned surfaces were investigated for neuronal cell growth with NG108-15 cells for short term culture and rat neural stem cells for longer term culture. The cells initially adhered highly selectively to the ablation tracks while longer term cell culture revealed a more uniform cell coverage of the surface.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Keywords: | laser ablation; diamond-like carbon; poly(oligo (ethylene glycol) methacrylate); atom transfer radical polymerization; neuronal cell culture |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Funding Information: | Funder Grant number Engineering and Physical Sciences Research Council EP/K002503/1; EP/I007695/1 Medical Research Council MR/L012669/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 14 Dec 2021 08:46 |
Last Modified: | 14 Dec 2021 08:46 |
Status: | Published |
Publisher: | Frontiers Media SA |
Refereed: | Yes |
Identification Number: | 10.3389/fmats.2021.756055 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:181553 |