Causier, B orcid.org/0000-0001-5942-7031, Hopes, T, McKay, M et al. (2 more authors) (2022) Plants Utilise Ancient Conserved Peptide Upstream Open Reading Frames in Stress-Responsive Translational Regulation. Plant, Cell and Environment, 45 (4). pp. 1229-1241. ISSN 0140-7791
Abstract
The regulation of protein synthesis plays an important role in the growth and development of all organisms. Upstream open reading frames (uORFs) are commonly found in eukaryotic messenger RNA transcripts and typically attenuate the translation of associated downstream main ORFs (mORFs). Conserved peptide uORFs (CPuORFs) are a rare subset of uORFs, some of which have been shown to conditionally regulate translation by ribosome stalling. Here, we show that Arabidopsis CPuORF19, CPuORF46 and CPuORF47, which are ancient in origin, regulate translation of any downstream ORF, in response to the agriculturally significant environmental signals, heat stress and water limitation. Consequently, these CPuORFs represent a versatile toolkit for inducible gene expression with broad applications. Finally, we note that different classes of CPuORFs may operate during distinct phases of translation, which has implications for the bioengineering of these regulatory factors.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | 5′-leader; 5′-UTR; abiotic stress; Arabidopsis; CPuORF; ribosome stalling; translation regulation; uORF |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 09 Dec 2021 12:16 |
Last Modified: | 14 Mar 2023 10:40 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/pce.14277 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:181387 |