Dou, Y, Routledge, MN, Gong, Y orcid.org/0000-0003-4927-5526 et al. (5 more authors) (2021) Efficacy of epsilon-poly-L-lysine inhibition of postharvest blue mold in apples and potential mechanisms. Postharvest Biology and Technology, 171. 111346. p. 111346. ISSN 0925-5214
Abstract
Penicillium expansum is a major postharvest fungal pathogen and is the causal agent of blue mold decay in apples. Epsilon-poly-l-lysine (ε-PL) is a naturally-occurring polypeptide that has strong antimicrobial activity. It is primarily used to suppress foodborne pathogens in bread, beverage, meat products, etc. The potential application of ε-PL in the management of fungal postharvest diseases of fruit, however, has not been explored. In the present study, the inhibitory effect of ε-PL against blue mold (P. expansum) disease of apples and its potential mechanism of action were investigated. Results indicated that 600 mg L−1 of ε-PL could effectively inhibit mycelial growth of P. expansum in apples. Concentrations of ε-PL > 200 mg L−1 also inhibited germination of conidia and germ tube growth of P. expansum in potato dextrose broth (PDB). The inhibitory effect increased with increasing concentrations of ε-PL concentration. Further studies indicated that the possible mechanisms involved of ε-PL inhibition of P. expansum included the activation of defense-related enzyme activity and gene expression in apple fruit tissues. This included polyphenol oxidase (PPO), catalase (CAT), peroxidase (POD) and phenylalanine ammonialyse (PAL). ε-PL stimulated the production of intracellular reactive oxygen species (ROS) and degraded the integrity of the cell wall and plasma membrane of conidia, resulting in the death of conidial spores of P. expansum or their germination.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 Elsevier B.V. All rights reserved. This is an author produced version of an article, published in Postharvest Biology and Technology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Apple; Blue mold; Induced resistance; Epsilon-poly-l-lysine; Membrane integrity; ROS |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Chemistry and Biochemistry (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) > Clinical & Population Science Dept (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 23 Nov 2021 14:58 |
Last Modified: | 21 Mar 2022 01:38 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.postharvbio.2020.111346 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:180561 |
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Filename: Dou Y et al, Revised manuscript PBT.pdf
Licence: CC-BY-NC-ND 4.0