Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division

Abstract

This protocol details the isolation and in vitro maintenance of single hematopoietic stem cells (HSCs) in the absence of the bone marrow niche. The HSCs do not divide over 7 days and fully retain their long-term functional capacity in transplantation assays. Following hibernation culture, HSCs can be used to study quiescence exit and can be genetically manipulated as single cells prior to division. For complete details on the use and execution of this protocol, please refer to Oedekoven et al. (2021).

Metadata

Item Type:	Article
Authors/Creators:	Belmonte, Miriam Kent, David G https://orcid.org/0000-0001-7871-8811
Copyright, Publisher and Additional Information:	© 2021 The Authors
Keywords:	Animals,Cell Culture Techniques/methods,Cell Division,Cells, Cultured,Culture Media,Hematopoietic Stem Cells/cytology,Mice,Single-Cell Analysis
Dates:	Published: 17 December 2021 Published (online): 1 November 2021 Accepted: 8 October 2021
Institution:	The University of York
Academic Units:	The University of York > Faculty of Sciences (York) > Biology (York)
Depositing User:	Pure (York)
Date Deposited:	05 Nov 2021 09:10
Last Modified:	16 Oct 2024 17:58
Published Version:	https://doi.org/10.1016/j.xpro.2021.100927
Status:	Published
Refereed:	Yes
Identification Number:	10.1016/j.xpro.2021.100927
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:180075