Van Eeckhoven, J, Horsburgh, GJ, Dawson, DA et al. (3 more authors) (2022) Development of a multiplex microsatellite marker set for the study of the solitary red mason bee, Osmia bicornis (Megachilidae). Molecular Biology Reports, 49 (1). pp. 783-788. ISSN 0301-4851
Abstract
Background
Solitary bees, such as the red mason bee (Osmia bicornis), provide important ecosystem services including pollination. In the face of global declines of pollinator abundance, such haplodiploid Hymenopterans have a compounded extinction risk due to the potential for limited genetic diversity. In order to assess the genetic diversity of Osmia bicornis populations, we developed microsatellite markers and characterised them in two populations.
Methods and results
Microsatellite sequences were mined from the recently published Osmia bicornis genome, which was assembled from DNA extracted from a single male bee originating from the United Kingdom. Sequences were identified that contained dinucleotide, trinucleotide, and tetranucleotide repeat regions. Seventeen polymorphic microsatellite markers were designed and tested, sixteen of which were developed into four multiplex PCR sets to facilitate cheap, fast and efficient genotyping and were characterised in unrelated females from Germany (n = 19) and England (n = 14).
Conclusions
The microsatellite markers are highly informative, with a combined exclusion probability of 0.997 (first parent), which will enable studies of genetic structure and diversity to inform conservation efforts in this bee.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2021. This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/) |
Keywords: | Megachilidae; Red mason bee; Osmia bicornis; Microsatellite marker; Population structure; Conservation genetics |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Funding Information: | Funder Grant number EU - European Union 752656 |
Depositing User: | Symplectic Publications |
Date Deposited: | 05 Nov 2021 15:47 |
Last Modified: | 25 Jun 2023 22:48 |
Status: | Published |
Publisher: | Springer |
Identification Number: | 10.1007/s11033-021-06796-x |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:179915 |
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