Sarshar, S, Sendker, J, Qin, X et al. (6 more authors) (2018) Antiadhäsive In-vitro- und In-vivo-Wirkungen von hydroalkoholischem Extrakt aus Selleriefrüchten (Apium graveolens) gegen uropathogene E. coli [Antiadhesive hydroalcoholic extract from Apium graveolens fruits prevents bladder and kidney infection against uropathogenic E. coli]. Zeitschrift für Phytotherapie, 39 (2). pp. 57-62. ISSN 0722-348X
Abstract
Fruits from Apium graveolens (celery) are used traditionally in Persian and European medicine for the treatment of uncomplicated urinary tract infections. No data are available on A. graveolens extract on the interplay between uropathogenic E. coli and the eukaryotic host cells and on quorum sensing of the bacteria. The present study aimed to characterize an antiadhesive and anti quorum sensing effect of a characterized A. graveolens extract by specific in vitro assays and to correlate these effects with in vivo data obtained by an animal infection model. Hydroalcoholic extract CSE (EtOH-water, 1:1) from A. graveolens fruits was characterized by UHPLC/+ESI-QTOF-MS and investigated on antiproliferative activity against UPEC (strain NU14) and human T24 bladder cells. Antiadhesive properties of CSE were investigated within two different in vitro adhesion assays. For in vivo studies BALB/c mice were used in an UPEC infection model. The effect of CSE on bacterial load in bladder tissue was monitored within a 4- and 7 days pretreatment (200, 500 mg/kg) of the animals.
CSE was dominated by the presence of luteolin-glycosides and related flavones besides furocoumarins. CSE had no cytotoxic effects against UPEC and bladder cells. CSE exerts a dose dependent antiadhesive activity against UPEC strains NU14 and UTI89. CSE inhibited in a concentration-dependent manner bacterial quorum sensing. 4- and 7-day pretreatment of animals with CSE transurethrally infected with UPEC NU14, significantly reduced the bacterial load in bladder tissue.
CSE is assessed as an antiadhesive extract for which the traditional use in phytotherapy for UTI is justified.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Apium graveolens L.; adhesion; bladder cells; celery fruits; uropathogenic E. coli |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Chemistry and Biochemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 05 Nov 2021 10:53 |
Last Modified: | 05 Nov 2021 10:53 |
Status: | Published |
Publisher: | Georg Thieme Verlag |
Identification Number: | 10.1055/a-0540-4929 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:179864 |