Bat-Erdene, Undramaa, Billingsley, John M., Turner, William C. et al. (5 more authors) (2021) Cell-Free Total Biosynthesis of Plant Terpene Natural Products Using an Orthogonal Cofactor Regeneration System. ACS Catalysis. pp. 9898-9903. ISSN 2155-5435
Abstract
Here we report the one-pot, cell-free enzymatic synthesis of the plant monoterpene nepetalactol starting from the readily available geraniol. A pair of orthogonal cofactor regeneration systems permitted NAD+-dependent geraniol oxidation followed by NADPH-dependent reductive cyclization without isolation of intermediates. The orthogonal cofactor regeneration system maintained a high ratio of NAD+ to NADH and a low ratio of NADP+ to NADPH. The overall reaction contains four biosynthetic enzymes, including a soluble P450, and five accessory and cofactor regeneration enzymes. Furthermore, addition of a NAD+-dependent dehydrogenase to the one-pot mixture led to ∼1 g/L of nepetalactone, the active cat attractant in catnip.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Funding Information: This work is support by NIH R01AT010001 to Y.T. The plasmid used for expression of NoxE was a gift from Prof. James Bowie. © 2021 American Chemical Society. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details |
Keywords: | biocatalysis,cofactor regeneration,enzyme cascade,natural products,synthetic biochemistry |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Centre for Immunology and Infection (CII) (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 29 Oct 2021 14:30 |
Last Modified: | 23 Jan 2025 00:26 |
Published Version: | https://doi.org/10.1021/acscatal.1c02267 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1021/acscatal.1c02267 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:179781 |
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