AZD1222/ChAdOx1 nCoV-19 vaccination induces a polyfunctional spike protein-specific Th1 response with a diverse TCR repertoire.

Abstract

AZD1222 (ChAdOx1 nCoV-19), a replication-deficient simian adenovirus-vectored vaccine, has demonstrated safety, efficacy, and immunogenicity against coronavirus disease 2019 (COVID-19) in clinical trials and real-world studies. We characterized CD4+ and CD8+ T cell responses induced by AZD1222 vaccination in peripheral blood mononuclear cells (PBMCs) from 296 unique vaccine recipients aged 18 to 85 years who enrolled in the phase 2/3 COV002 trial. Total spike protein-specific CD4+ T cell helper type 1 (Th1) and CD8+ T cell responses were increased in AZD1222-vaccinated adults of all ages following two doses of AZD1222. CD4+ Th2 responses following AZD1222 vaccination were not detected. Furthermore, AZD1222-specific Th1 and CD8+ T cells both displayed a high degree of polyfunctionality in all adult age groups. T cell receptor (TCR) β sequences from vaccinated participants mapped against TCR sequences known to react to SARS-CoV-2 revealed substantial breadth and depth across the SARS-CoV-2 spike protein for both AZD1222-induced CD4+ and CD8+ T cell responses. Overall, AZD1222 vaccination induced a polyfunctional Th1-dominated T cell response, with broad CD4+ and CD8+ T cell coverage across the SARS-CoV-2 spike protein.

Metadata

Item Type:	Article
Authors/Creators:	Swanson, PA Padilla, M Hoyland, W McGlinchey, K Fields, PA Bibi, S Faust, SN McDermott, AB Lambe, T Pollard, AJ Durham, NM Kelly, EJ AstraZeneca/Oxford/VRC Study Group Adlou, S Aley, PK Angus, B Anslow, R Baker, P Bansal, H Beveridge, A Bridges-Webb, A Ching, S Cicconi, P Clutterbuck, EA Collins, AM Darton, TC https://orcid.org/0000-0003-2209-9956 Demissie, T Dinesh, T Douglas, AD Duncan, CJA Ewer, KJ Felle, S Ferreira, DM Folegatti, PM Fuskova, M Gaudinski, M Gilbert, SC Goodman, AL Gordon, I Green, CA Harbolick, E Hayes, S Hill, AVS Hill, H Jenkin, D Jepson, BM Kasanyinga, M Libri, V Lillie, PJ McGregor, AC Minassian, AM Mujadidi, YF Novik, L Payne, R Pilataxi, F Plested, E Provstgaard-Morys, S Ramasamy, M Robinson, H Sanders, K Smith, A Snape, MD Song, R Sutherland, RK Thomson, EC Toshner, M Turner, DPJ Voysey, M Widge, AT Williams, CJ
Copyright, Publisher and Additional Information:	© 2021 The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. This license does not apply to figures/photos/artwork or other content included in the article that is credited to a third party; obtain authorization from the rights holder before using this material.
Dates:	Published: 17 November 2021 Published (online): 30 September 2021
Institution:	The University of Sheffield
Academic Units:	The University of Sheffield > Sheffield Teaching Hospitals
Depositing User:	Symplectic Sheffield
Date Deposited:	11 Oct 2021 12:18
Last Modified:	09 Mar 2022 17:02
Status:	Published
Publisher:	American Association for the Advancement of Science
Refereed:	Yes
Identification Number:	10.1126/scitranslmed.abj7211
Related URLs:	PubMed URL Dataset
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:178925

CORE (COnnecting REpositories)

AZD1222/ChAdOx1 nCoV-19 vaccination induces a polyfunctional spike protein-specific Th1 response with a diverse TCR repertoire.

Abstract

Metadata

Download

Published Version

Export

Statistics

AZD1222/ChAdOx1 nCoV-19 vaccination induces a polyfunctional spike protein-specific Th1 response with a diverse TCR repertoire.

Abstract

Metadata

Download

Published Version

Related datasets

Export

Statistics