Derrick, C.J., Pollitt, E.J.G., Sevilla Uruchurtu, A.S. et al. (3 more authors) (2021) Lamb1a regulates atrial growth by limiting second heart field addition during zebrafish heart development. Development, 148 (20). dev199691. ISSN 0950-1991
Abstract
During early vertebrate heart development the heart transitions from a linear tube to a complex asymmetric structure, a morphogenetic process which occurs simultaneously with growth of the heart. Cardiac growth during early heart morphogenesis is driven by deployment of cells from the Second Heart Field (SHF) into both poles of the heart. Laminin is a core component of the extracellular matrix (ECM), and although mutations in laminin subunits are linked with cardiac abnormalities, no role for laminin has been identified in early vertebrate heart morphogenesis. We identified tissue-specific expression of laminin genes in the developing zebrafish heart, supporting a role for laminins in heart morphogenesis. Analysis of heart development in lamb1a zebrafish mutant embryos reveals mild morphogenetic defects and progressive cardiomegaly, and that Lamb1a functions to limit heart size during cardiac development by restricting SHF addition. lamb1a mutants exhibit hallmarks of altered haemodynamics, and blocking cardiac contractility in lamb1a mutants rescues heart size and atrial SHF addition. Together this suggests that laminin mediates interactions between SHF deployment and cardiac biomechanics during heart development and growth in the developing embryo.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 The Authors. This is an author-produced version of a paper subsequently published in Development. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Contractility; Extracellular matrix; Heart development; Laminin; Second heart field; Zebrafish |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Sheffield Teaching Hospitals |
Funding Information: | Funder Grant number British Heart Foundation FS/16/3732347 Rosetrees Trust A1252 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 06 Oct 2021 09:49 |
Last Modified: | 10 Mar 2022 17:04 |
Status: | Published |
Publisher: | The Company of Biologists |
Refereed: | Yes |
Identification Number: | 10.1242/dev.199691 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:178917 |
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Filename: Derrick et al Laminin Development 2021.pdf
