Miles, JA orcid.org/0000-0001-8839-9201, Egan, JL, Fowler, J et al. (5 more authors) (2021) The evolutionary origins of peroxynitrite signalling. Biochemical and Biophysical Research Communications, 580. pp. 107-112. ISSN 0006-291X
Abstract
Peroxynitrite is a reactive intermediate formed in vivo through uncatalysed reaction of superoxide and nitric oxide radicals. Despite significant interest in detecting peroxynitrite in vivo and understanding its production, little attention has been given to the evolutionary origins of peroxynitrite signalling. Herein we focus on two enzymes that are key to the biosynthesis of superoxide and nitric oxide, NADPH oxidase 5 (NOX5) and endothelial nitric oxide synthase (eNOS), respectively. Multiple sequence alignments of both enzymes including homologues from all domains of life, coupled with a phylogenetic analysis of NOX5, suggest eNOS and NOX5 are present in animals as the result of horizontal gene transfer from ancestral cyanobacteria to ancestral eukaryotes. Therefore, biochemical studies from other laboratories on a NOX5 homologue in Cylindrospermum stagnale and an eNOS homologue in Synechococcus sp. PCC 7335 are likely to be of relevance to human NOX5 and eNOS and to the production of superoxide, nitric oxide and peroxynitrite in humans.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 Elsevier Inc. All rights reserved. This is an author produced version of an article, published in Biochemical and Biophysical Research Communications Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Peroxynitrite; NADPH oxidase 5; Nitric oxide synthase; Cyanobacteria; Multiple sequence alignment; Phylogenetic analysis |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Oct 2021 14:03 |
Last Modified: | 30 Sep 2022 00:18 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.bbrc.2021.09.071 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:178727 |