Wilson, LA, Heraty, L, Ashford, BA et al. (5 more authors) (2021) Tissue microarray (TMA) use in post mortem neuropathology. Journal of Neuroscience Methods, 347. 108963. ISSN 0165-0270
Abstract
Background
Tissue microarrays (TMAs), where each block (and thus section) contains multiple tissue cores from multiple blocks potentially allow more efficient use of tissue, reagents and time in neuropathology.
New method
The relationship between data from TMA cores and whole sections was investigated using ‘virtual’ TMA cores. This involved quantitative assessments of microglial pathology in white matter lesions and motor neuron disease, alongside qualitative TDP-43 inclusion status in motor neuron disease cases. Following this, a protocol was developed for TMA construction.
Results
For microglial pathology we found good concordance between virtual cores and whole sections for volume density using one 1.75 mm core (equivalent to a 2 mm core after accounting for peripheral tissue loss). More sophisticated microglial cell size and measures required two cores. Qualitative results of pTDP-43 pathology showed use of one 1.75 mm core gave a 100 % sensitivity and specificity within grey matter, and 88.3 % sensitivity and 100 % specificity within white matter. A method of producing the TMAs was suitable for immunohistochemistry both manually and by autostainer, with the minimal core loss from the microscope slide.
Comparison with existing methods
TMAs have been used infrequently in post mortem neuropathology research. However, we believe TMAs give comparable tissue assessment results and can be constructed, sectioned and stained with relative ease.
Conclusions
We found TMAs could be used to assess both quantitative (microglial pathology) and qualitative pathology (TDP-43 proteinopathy) with greatly reduced quantities of tissue, time and reagents. These could be used for further work to improve data acquisition efficiency.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 Elsevier B.V. All rights reserved. This is an author produced version of an article published in Journal of Neuroscience Methods. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Tissue microarray; Neuropathology; Post mortem; Neurodegeneration; Formalin fixed paraffin embedded; Immunohistochemistry |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Computing (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 30 Sep 2021 11:46 |
Last Modified: | 25 Jun 2023 22:46 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.jneumeth.2020.108963 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:178617 |
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Filename: Final Accepted Manuscript - TMA Use in PM Neuropathology.pdf
Licence: CC-BY-NC-ND 4.0